马立克病病毒与禽网状内皮增生病病毒的混合感染及在传代过程中的重组

doi:10.11843/j.issn.0366-6964.2016.01.017

Abstract

Abstract:

This study was performed to investigate the co-infection and natural recombination of Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV) in chicken flocks in China，and evaluate the potential influences of viral recombination on MDV biology.Based on polymerase chain reaction (PCR)，DNA sequencing and indirect immunofluorescent assay (IFA)，we presently studied a total of 17 MDV field isolates obtained from the vaccinated chicken flocks in Henan province.The PCR results showed that，among the 17 Henan isolates，two isolates，HNGS206 and HNXZ103，are recombinant MDVs containing the REV－LTR inserts.Subsequent experiments showed that neither of the two isolates are natural recombinant viruses and the integrations of LTR into the viral genomes may result from the clinical co-infection and/or virus isolation procedure.Our data indicates that although a wide-spread co-infection of both MDV and REV exists in chicken flocks in China，the epidemiology of the naturally occurring recombinant MDVs needs to be further monitored.

CLC Number:

S858.315.3

SU Jing-wei，TENG Man，LUO Jun，CHI Jia-qi，YU Zu-hua，YU Le-le，HU Bo，ZHANG Gai-ping. Co-infection of Field Marek’s Disease Virus with Reticuloendotheliosis Virus Prevalent in Vaccinated Chicken Flocks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 128-134.

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Co-infection of Field Marek’s Disease Virus with Reticuloendotheliosis Virus Prevalent in Vaccinated Chicken Flocks

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